TALES TO ASTONISH #39 (1963): 1st Scarlet Beetle

Radiation makes a bug big and smart, and Ant-Man fights it. 

In the end, Hank is able to counter the radiation, re-shrink the beetle to beetle-size, and return him to his own garden.

Believe it or not, the Scarlet Beetle will appear in several more Ant-Man stories.

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